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Effects of Anthrax Toxins on
Actin Based Motility |
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In collaboration with the CDC we are about to
begin investigations of the effects of the anthrax toxins. Protective
antigen combined with Lethal and Edema toxin on actin-based motility of
macrophages and neutrophils. By paralyzing the innate immune system anthrax
is able to rapidly invade the host and cause fatal disease. Lethal and Edema
toxin are able to enter the cytoplasm of cells when combined with protective
antigen, and have previously been shown to affect neutrophil chemotaxis and
phagocytosis, two motile processes that require major rearrangements of the
cytoskeleton. Using FACs analysis, time lapse video and specific actin
filament staining we will examine how these toxins affect the actin
cytoskeleton. |
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